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KMID : 0360319930250060848
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Journal of Korean Cancer Research Association
1993 Volume.25 No. 6 p.848 ~ p.854
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Recessive Allelic Loss in Colorectal Cancer Tissue
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조원일
유승진/장석균/김승남/송영택/이재학/주상용
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Abstract
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Two types of genetic alterations have been reported in colorectal tumors. The first type involves point mutations in ras proto-oncogenes. The second type of alterations involves deletion of specific chromosomal regions. Deletions can be detected
by
restriction fragment lenght polymorphism analyisis of tumor DNA. The deleted sequences have been hypothesized to include tumor suppressor gene. The inactivation of tumor-suppressor gene by deletion of suspected locus lead to neoplatic growth. To
investigate the relation between ras point mutation and allelic deletion of chromosome 17p and 18p, both p-PCR and RFLP analysis using VNTR marker and synthetic oligonulectide probe tailing with digoxigenin 11-dUTP were done in 15 normal mucosa
and
22
colorectal cancer mucosa.
@EN 1) Incidence of ras point mutations was 80%(4/5) in Dukes' B, 76.4%(13/17) in Dukes' C. There was increasing tendency of mutation along with stage progression, but no statistical significance was noted.
2) Analysis of incidence of mutations over 2 points in the same DNA sequence between well differentiated and moderately differentiated cancer did pot show any statistical significance.
3) Incidence of allelic deletion of 17p pYNH37 WERE 60%(3/5) in Dukes' B, 46.7%(11/17) in Dukes' C. There was no correlation of incidences of 18p OS-4 in colorectal cancer. But incidence of 17p allelic deletion was higher than 18p allelic
deletion in
Dukes' C colorectal cancer. The allelic deletions of pYNZ22 were noted in 6 cases of Dukes' C colorectal cancer but not in dukes' B colorectal cancer.
4) There was no statistical difference between incidence of ras point mutation and allelic deletion in colorectal cancer.
These results showed there was no statistical difference in incidence of ras point mutation & 18p allelic deletion in colorectal cancer. 17p allelic deletion occurred more frequently in advanced colorectal cancer. Genetic alteration did not have
any
relation with cellular differentiation or tumor location in the biologic behavior of the colorectal cancer.
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KEYWORD
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